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| Publication year: 2010 Source: Biomaterials, Volume 31, Issue 31, November 2010, Page IFC [No author name available] |
| Publication year: 2010 Source: Biomaterials, In Press, Corrected Proof, Available online 25 August 2010 Zi-Xian, Liao , Yi-Cheng, Ho , Hsin-Lung, Chen , Shu-Fen, Peng , Chun-Wen, Hsiao , ... Although advantageous for siRNA packing and protection, chitosan (CS)-based complexes may lead to difficulties in siRNA release once they arrive at the site of action, due to their electrostatic interactions. To assist the intracellular release of siRNA and thus enhance its effectiveness in gene silencing, we incorporated a negatively charged poly(γ-glutamic acid) (γ-PGA) into CS/siRNA complexes. The inclusion of γ-PGA did not alter the complex-formation ability between CS and siRNA; additionally, their cellular uptake was significantly enhanced. The results obtained in our molecular dynamic simulations indicate that the binding between CS and siRNA remained stable in the cytosol environment. In... |
| Publication year: 2010 Source: Biomaterials, In Press, Corrected Proof, Available online 25 August 2010 Arlin L., Weikel , Steven G., Owens , Nicole L., Morozowich , Meng, Deng , Lakshmi S., Nair , ... The preparation of phosphazene tissue engineering scaffolds with bioactive side groups has been accomplished using the biological buffer, choline chloride. Mixed-substituent phosphazene cyclic trimers (as model systems) and polymers with choline chloride and glycine ethyl ester, alanine ethyl ester, valine ethyl ester, or phenylalanine ethyl ester were synthesized. Two different synthetic protocols were examined. A sodium hydride mediated route resulted in polyphosphazenes with a low choline content, while a cesium carbonate mediated process produced polyphosphazenes with higher choline content. The phosphazene structures and physical properties were studied using multinuclear NMR, differential scanning calorimetry (DSC), and gel permeation chromatography (GPC) techniques.... |
| Publication year: 2010 Source: Biomaterials, In Press, Corrected Proof, Available online 25 August 2010 Aase, Bodin , Shantaram, Bharadwaj , Shaofeng, Wu , Paul, Gatenholm , Anthony, Atala , ... The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (106 cells/cm2) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin... |
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